Chapter 60 Season 2

Second Seal: Tsukune uses even more power in second seal of his Holy Lock and is on par with Gyokuro using her own seal in this form. Tsukune's speed and power increases dramatically in this form according to Gyokuro his fighting potential is equal to Alucard's. Tsukune could easily beat numerous foes in the R+V universe using second seal. The fact that electiricity and heat did not phase Tsukune in this form shows.The power of the Shinso blood will take over his body and Tsukune's sanity  becomes closer to a second ghoul form by using second seal.